Anatomy and Physiology IV

DeVry_University_Chicago_-_BIOS_256_BIOS256_W3_PowerPhys2_Lab_Report-2.docx-BIOS256 Week 3 Lab: #3 Enzyme

BIOS256 Week 4 Lab: #10 Inﬂuence of Fluid Intake on Urine Formaon Laboratory Report Acvity 10: Inﬂuence of Fluid Intake on Urine Formaon Name: Shamir Tyson Instructor: Professor Shen Date: November 24, 2013 PREDICTIONS 1. Urine output will be highest during: Water Loading 2. Urine osmolality will be lowest during: Dehydraon 3. Plasma osmolality: increases with dehydraon METHODS AND MATERIALS 1. Dependent variable: Urine and plasma values 2. Independent variable: ﬂuid intake 3. Controlled variables: age, gender and weight/ Room temperature 4. Subjects were asked to refrain from alcohol and caﬀeine for a day before the start of the experiment. Explain why this is important. This is important as caﬀeine and alcohol could aﬀect the outcome of the experiment especially these products could cause excreon of urine since they are diurecs and eﬀect plasma work. 5. What technique was used to measure urine and plasma osmolality? The centrifuge was used to separate the plasma. The plasma and urine was then refrigerated and later accessed and placed in the osmometer for results RESULTS See Table 3: Urine Producon Rate See Graph 1: Average Daily Urine Producon Under Diﬀerent Hydraon States Type in the following AVERAGE urine producon rate (L/day)  Normal  Dehydrated  Water Loaded Normal Dehydrated Water Loaded Subject 1 1.98 0.63 7.59 Subject 2 1.96 0.62 7.18 Subject 3 1.82 0.64 7.48 Average 1.92 0.63 7.42 1. Does dehydraon increase, decrease, or not change average urine producon rate (L/day)? Dehydraon decreases urine producon 2. Does water loading increase, decrease, or not aﬀect average urine producon rate (L/day)? Water loading increases the urine producon rate See Table 4: Osmolality (mosm/L) See Graph 2: Blood Plasma and Urine Osmolality Under Diﬀerent Hydraon States Type in the following AVERAGE urine osmolality (mosm/L)  Normal  Dehydrated  Water Loaded Urine Normal Dehydrated Water Loaded Subject 1 630 1250 188 Subject 2 585 1180 188 Subject 3 621 1170 162 Average 612 1200 179 3. Does dehydraon increase, decrease, or not change average urine osmolality (mosm/L)? Dehydraon increases urine osmolality 4. Does water loading increase, decrease, or not change average urine osmolality (mosm/L)? Water loading decreases urine osmolality Type in the following AVERAGE plasma osmolality (mosm/L)  Normal  Dehydrated  Water Loaded Plasma Normal Dehydrated Water Loaded Subject 1 304 307 283 Subject 2 295 306 270 Subject 3 268 308 273 Average 295 307 275 5. Does dehydraon increase, decrease, or not change average plasma osmolality (mosm/L)? Dehydraon slightly increases plasma osmolality 6. Does water loading increase, decrease, or not change average plasma osmolality (mosm/L)? Water loading decreases osmolality DISCUSSION 1. State whether dehydraon results in producon of a concentrated or dilute urine. Dehydraon results in producon of concentrated Urine 2. State whether water loading results in producon of concentrated or dilute urine. Water loading results in dilute urine 3. Describe how ADH secreon during dehydraon changes urine producon and osmolality. ADH secreon changes urine producon by aiding in the change of reabsorpon. When you are dehydrated, you will release more ADH, which will reabsorb water, making you have more blood volume and lower osmolality.

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