Microbiology

Micro_Exam_2_Study_Guide.docx.docx-MICRO EXAM 2 STUDY GUIDE Quiz

Quiz 1- Lab Safety & Antiseptic Procedures How long should an inoculating instrument be held in the bacti-incinerator? About 5-10 seconds or until loop/needle glows red (red hot) agar plate In most cases, how is this type of media incubated? Inverted (upside down) Wipe bench-top with disinfectant at the beginning and end of each lab. True Tie back shoulder-length or longer hair to prevent potential fire hazards. True Which of the following best describes how a culture tube is handled for aseptic transfer? Holding only one tube at a time and at an angle to obtain the inoculum. Inserting only the portion of the loop or needle that got red hot in the bacti-incinerator. What biosafety level (BSL) are the organisms used in our lab? BSL 1 Open toe and open heel shoes are permitted in the micro lab. False All test tubes must be carried in a test tube rack. True Place contaminated instruments on the bench-top to prevent cross-contamination of other sterile instruments. False A test tube containing a liquid medium is referred to as: Broth This type of medium is referred to as: An agar slant When using a bacti-incinerator… Keep any/all flammables clear of device Do not leave unattended Aseptic Technique is A method of preventing unwanted microbes from gaining access This inoculating tool is called: A loop Place in order the correct sequence of safety procedures upon entry into the lab 1.Place personal items in designated area 2.Wash hands using liquid detergent and dry with paper towels 3.Disinfect bench tops 4.Prepare to work; have pages of lab exercise out and ready to take notes The streak plate technique is used to isolate a pure colony of bacteria from a mixed population so that subcultures can be made for further study and identification. True Which of the following information must be written when labeling cultures? Name of microbe, if known or source when sample was collected Student or group name Date Which of the following is the correct location to dispose of contaminated items? The red (or orange) biohazard containers Your lab exercise provides examples of several types of streak plates. Which of the following type of streak plate does Dr. Kaiser demonstrate in the video? T streak You may bring your laptop, iPad, or other electronic devices to your bench so that you can take notes during the lab experiment. You can also bring your cell phone to take pictures. False Quiz 2 Smears, Stains, and Scopes Which of the following smear preparations require the use of water? Smears from an agar slant Smears from an agar plate Match the stain with the most appropriate description Primary stain- Crystal violet Mordant- Iodine Decolorization- Alcohol/ acetone Counterstain- Safranin When preparing a smear, the loop should be sterilized (incinerated) before and after use. True The purpose of heat fixation is: To coagulate proteins and “melt” lipids in order to adhere bacteria to the slide

BIOL | 2230 | Microbiology | study guides | Quiz 1- Lab Safety & Antisepti

MICRO_LECTURE__Exam_1_STDY_G.docx.docx-Chapter 1 & 4 Quiz In

MICRO EXAM 3 STUDY GUIDE Quiz 5: Chapters 7 and 20 Which of the following is NOT considered a mode of action by antimicrobial agents? Disruption of selective toxicity The mode of action of an antimicrobial agent refers to How it exerts its effects on the microbe Diarrhea, yeast infections, and superinfection are associated with which of the following? Broad spectrum antibiotics Microbial death has occurred if Reproductive capability has been lost even when placed in optimal conditions Which part of the cell envelope restricts the passage of many drugs into gram-negative bacteria? Outer membrane Upon entering the lab and upon completion of the lab, you are required to clean the bench top: this process is… Disinfection Antibiotics used in the treatment of bacterial infections target the differences between the host (us) and the pathogen (them). One target of particular importance are the ribosomes. Choose the statement that correctly describes the major difference between prokaryotic and eukaryotic ribosomes. Prokaryotes and eukaryotes ribosomes differ in size Why are antibiotics that inhibit protein synthesis in bacterial cells not effective in inhibiting protein synthesis in yeast? Fungi are eukaryotic cells with 80S ribosomes Which of the following microbial forms have the highest resistance to physical and chemical controls? Bacterial endospores Which of the following antibiotics is rendered ineffective from the production of the beta- lactamase? Penicillin G Based on the results below, which compound was the most effective against Staphylococcus using the disk-diffusion test: Disinfectant Zone of Inhibition (mm) A 0 B 2.5 C 10 D 5 Disinfectant C You find work in a pharmaceutical lab conducting experiments on the mode of actions of antimicrobial agents very rewarding. As you conduct experiments on Chloramphenicol, you note that it inhibits the formation of the growing polypeptide chain. Therefore the target of this antimicrobial agent must be the _______. 50S Ribosomal subunit The phase of the bacterial growth curve in which the rate of multiplication equals the rate of cell death is the _________. Stationary phase An autoclave sterilizes through the use of Moist heat Under which condition would alcohol be most effective? By using water to lower the concentration from 100% to 70% A bacteriostatic agent is one that ____________. Inhibits the growth of the microbe A new health care worker was asked to retrieve a chemical agent that can be safely used externally on living tissue. Which of the following is the most appropriate chemical agent she should use? Antiseptic One way to overcome resistance to penicillin is to combine penicillin with potassium clavulanate, that protects penicillin. Potassium clavulanate is an __________. Beta-lactamase inhibitor

BIOL | 2230 | Microbiology | study guides | MICRO EXAM 3 STUDY GUIDE Quiz 

MICRO_LAB_EXAM_2.docx.docx-LAB EXAM 2 STUDY GUIDE Quiz

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