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Bacterial Isolation assignment MS.docx.docx
Bacterial_Isolation_assignment_MS.docx.docx
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Bacterial Isolation assignment MS.docx.docx-Macken...
Bacterial_Isolation_assignment_MS.docx.docx-Mackenzie Strang Homework Quesons: 10 points
Bacterial Isolation assignment MS.d...
Bacterial_Isolation_assignment_MS.docx.docx-Mackenzie Strang Homework Quesons: 10 points
Page 1
Mackenzie Strang
Homework Quesons: 10 points
1.How did Max most likely get sick and what was the source of the pathogen?
-Max had chicken for dinner the night before he got sick. Chickens feces at the farm were found
to carry anbioc resistant
E. coli
and
Salmonella
most likely from the amount of anbiocs in
the chicken’s food. The doctor menoned that he did not believe the chicken was cooked
thoroughly.
2. What type of agar are we using to culture? Is this agar selecve, differenal, and/or complex?
-The plate used to culture was
SalmonellaShigella
agar (SSA). This agar is selecve because it
contains selecve agents that only allow the growth of certain gram-negave bacterial strains
and prevent the growth of gram-posive strains.
3. What color do Salmonella appear on this agar and why?
-
Salmonella
appear black on the SSA plate because most
Salmonella
strains can reduce
thiosulfate and produce hydrogen sulfide, which reacts with the ferric acid in the medium,
making it black.
4. What color do E. coli appear on SSA and why?
-
E. coli
appear red on the SSA plate because the medium contains the pH indicator neutral red. It
becomes red when the pH is below 6.8.
E. coli
can cause this change because it is a lactose
fermenng bacterium that converts lactose to lacc acid.
5. Bunsen burners are used extensively in microbiology.
Why is it important to work in close proximity
to a Bunsen burner in order to minimize contaminaon?
-The flame and heat from the Bunsen burner keep away and kill microorganisms that are in the
air, which prevent the plate from being contaminated and overrun with other organisms.
6.
Why was Max’s infecon so hard to treat?
-Max’s infecon was caused by an anbioc resistant strain of
Salmonella
, which means that
any anbiocs he was treated with that would normally work on a bacterial infecon, were not
having any effect.
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