BIOS | 256 | Anatomy and Physiology IV | lab | BIOS256 Week 3 Lab: #3 Enzyme

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DeVry_University_Chicago_-_BIOS_256_BIOS256_W3_PowerPhys2_Lab_Report-2.docx-BIOS256 Week 3 Lab: #3 Enzyme

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BIOS256 Week 3 Lab: #3 Enzyme Acvity Laboratory Report

Acvity 1:

Enzyme Acvity

Name: Shamir

Instructor: Professor Shen

Date: 11/14/13

PREDICTIONS

1. Sucrase will have the greatest acvity at: ph of 7

2. Sucrase will have the greatest acvity at: 10c

3. Sucrase acvity: does not change with increase of sucrose

MATERIALS AND METHODS

Eﬀect of pH on Enzyme Acvity

1. Dependent Variable:

Amount of product used

2. Independent Variable: PH

3.Controlled Variables: Sucrose and sucrose incubaon me, Amount of Substrate used,

Eﬀect of Temperature on Enzyme Acvity

1. Dependent Variable: Amount of product used (glucose an fructose)

2. Independent Variable: Temperature

3. Controlled Variables; Sucrose and sucrose incubaon me, Amount of Substrate used,

Eﬀect of Substrate Concentraon on Enzyme Acvity

1. Dependent Variable: Amount of product used (glucose and fructose

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2. Independent Variable: Amount of substrate (sucrose) present

3. Controlled Variables: Temperature, incubaon me and PH

4. Describe what is measured as an indicator of sucrase acvity and why this is an

indicator of sucrase acvity.

Opmal density is measured because it measure the

amount of glucose and fructose produced

5. Explain why denatured sucrase was used as a control.

Because Denatured Sucrose will not have as much acvity as the other sucrose

6. Addion of DNS at the end of the incubaon period stopped the reacon by

denaturing sucrase. Explain why it is important to denature sucrase before measuring

product concentraon. In order too decrease enzyme acvity

RESULTS

See Table 1: Eﬀect of pH on Sucrase Acvity

See Graph: Eﬀect of pH on Sucrase Acvity

1. State the opmum pH for sucrase acvity, and describe how sucrase acvity changes

at more acidic and more alkaline pH values. The opmum PH for sucrose is a PH OF 6

with an opmal density of 0.933

See Table 2: Eﬀect of Temperature on Sucrase Acvity

See Graph: Eﬀect of Temperature on Sucrase Acvity

2. Was the rate of increase of sucrase acvity higher at a pH of 8.5 or a pH of 5.5?

PH of 5.5

3. State the opmum temperature for sucrase acvity, and describe how sucrase acvity

changes at lower and higher temperatures.

40 degrees Celsius. Sucrase acvity decreases with the higher temp

See Table 3: Eﬀect of Sucrose Concentraon on Sucrase Acvity

See Graph: Eﬀect of Sucrose Concentraon on Sucrase Acvity

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4. Was sucrase acvity higher at 25 ºC or 55 ºC? 55c

5. State how sucrase acvity changes with increasing sucrose concentraon. As sucrose

concentraon increases the lower the acvity.

6. Was the rate of increase of sucrase acvity greater when sucrose concentraon went

from 2.5 to 7.5 g/l or when it went from 22.5 to 27.5 g/l? 2.5 to 7.5 G/L

pH Along the GI Tract

Organ

Mouth

6.35 – 6.85

Stomach

1 – 3

Duodenum (ﬁrst secon of small intesne)

Jejuneum (second secon of small intesne)

7.5

Ileum (third secon of small intesne)

7.5

Colon

7.5 – 8

DISCUSSION

1. Referring to the table above, speciﬁcally state where in the intesne sucrase is

likely most

acve. Duodenum

2. Compare opmal temperature for sucrase acvity to body temperature.

Sucrose acvity is more acve when it closer to the body’s temperature

How would a slight fever aﬀect sucrase acvity? It would increase sucrose acvity

4. Explain how pH of intesne and temperature of body together aﬀect enzyme

acvity. Both the PH of the intesnes and temperature of the body result in higher

sucrose acvity

5. Explain how increasing amount of sucrose in food aﬀects sucrase acvity.

Increasing the amount of sucrose in food would result in greater sucrose acvity, as the

products of glucose and fructose are higher

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