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Keiser University Port Saint Lucie - MICROBIOLO 101 Virtual ...
Keiser_University_Port_Saint_Lucie_-_MICROBIOLO_101_Virtual_Lab_1_Worksheet_Bianca_Caroll.doc
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Keiser University Port Saint Lucie - MICROBIOLO 10...
Keiser_University_Port_Saint_Lucie_-_MICROBIOLO_101_Virtual_Lab_1_Worksheet_Bianca_Caroll.doc-Keiser University Online Microbiology I Virtual
Keiser University Port Saint Lucie ...
Keiser_University_Port_Saint_Lucie_-_MICROBIOLO_101_Virtual_Lab_1_Worksheet_Bianca_Caroll.doc-Keiser University Online Microbiology I Virtual
Page 1
Keiser University Online Microbiology I
Virtual Lab 1 Worksheet
Microscopy & Gram Staining
Name:
_________
1.
For each of the 4 microscopes below, list some items that you would be able to
view with that particular type of microscope.
Compound Light
:
Tissue Samples, Blood
and Microscopic Cells
SEM
:
The details of a specimens surface
Dissecting Scope
:
Organs, Tissue,
Leaves, Stems and Mold
TEM
:
Internal structures of a cell
2.
For the letter “e”, draw or describe what it looked like under the objectives below:
4X
10X
40X
3.
After you become familiar with the microscope and view the slides, then try your
hand at the puzzles and measuring exercises.
How did you adjust the scope to
come into crisp focus
?
What do you see
?
How large are the objects in
m
?
Write your answers below:
P1=
Adjusted course focus,
fine focus, centered image,
placed 40X lens. I am able
to see a line from part of
the letter “e”.
P4=
Adjust iris diaphragm,
centered image, adjusted
course focus and fine
focus. I can see the onion
root tip.
M1=
I had to rotate the
micrometer from top to
bottom. 145 Micrometers
P2=
Adjusted iris
diaphragm, adjusted course
and fine focus and centered
specimen. Looks like black
dots in little boxes.
P5=
Adjust diaphragm,
course and fine focus and
center image. Pink
background with little flakes
of white.
M2=
I had to move the XY
controls to get the image
right next to the
micrometer. 8.0
Micrometers
P3=
Centered image,
adjusted course and fine
P6=
Adjusted Course
Focus and fine focus, XY
M3=
I had to move the XY
controls to get the image
1
Page 2
Keiser University Online Microbiology I
Virtual Lab 1 Worksheet
Microscopy & Gram Staining
focus. Shows blue shapes
with dark blue specks in the
middle.
Controls to center image. I
see bluish shapes with a
dark blue dot in the center.
right next to the
micrometer. 2 Micrometers
4.
Label the above components of the compound light
microscope:
A.
Ocular Lens
F.
Rheostat
B.
Stage (XY) Controls
G.
Spring Loaded Stage Clip
C.
Course Focus
H.
Illuminator
D.
Fine Focus
I.
Iris Diaphragm
E.
Lenses
5.
Define the following:
a.
magnification
The seeming increase in size of an object viewed under the
microscope
b.
resolution
The power to show details clearly. The optic ability to
2
Page 3
Keiser University Online Microbiology I
Virtual Lab 1 Worksheet
Microscopy & Gram Staining
distinguish detail such as the separation of closely adjacent
objects.
c.
contrast
converts phase shifts in light passing through a transparent
specimen to brightness changes in the image
d.
ocular micrometer
A glass disk that fits in a microscope eyepiece and that has
a ruled scale; when calibrated with a slide micrometer, direct
measurements of a microscopic object can be made.
6.
Complete the following table:
Objective magnification
Ocular magnification
Total magnification
10X
10X
100X
45X
10X
450X
100X
10X
1000X
75X
10X
750X
45X
5X
225X
7.
While looking through the 40X objective lens, you use the micrometer and measure
an insect’s leg.
It is 16 mm.
What is the actual size of the insect leg?
The actual size of the insect’s leg is 40 um.
16 ocular micrometer units x 2.5 Calibration factor =
40 um ocular micrometer
8.
Explain why the light microscope is also called the compound microscope.
It is composed of two lens systems.
One lens system, called the objective, is placed
close to the specimen and produces an enlarged and resolved image of the specimen.
This image is in turn enlarged by another lens system, called the eyepiece or ocular,
which is placed near to the eye of the observer.
9.
Explain why the specimen must be centered in the field of view on low power before
going to high power
High power takes in a much smaller area, you may not be able to find the specimen if it
is off to one side. The entire field of view may be in the blank area of the slide.
10.
Describe how to make a proper wet mount of the letter “e”
Use the eyedropper to place a drop of water on the center of a microscope slide
Use the tweezers to position the letter "e" in the center of the drop.
3
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